@article{brodin_establishment_2016,
 abstract = {HIV-1 infection cannot be cured because the virus persists as integrated proviral DNA in long-lived cells despite years of suppressive antiretroviral therapy (ART). In a previous paper (Zanini et al, 2015) we documented HIV-1 evolution in  10 untreated patients. Here we characterize establishment, turnover, and evolution of viral DNA reservoirs in the same patients after 3-18 years of suppressive ART. A median of 14\% (range 0-42\%) of the DNA sequences were defective due to G-to-A hypermutation. Remaining DNA sequences showed no evidence of evolution over years of suppressive ART. Most sequences from the DNA reservoirs were very similar to viruses actively replicating in plasma (RNA sequences) shortly before start of ART. The results do not support persistent},
 area = {hiv_evolution},
 author = {Brodin, Johanna and Zanini, Fabio and Thebo, Lina and Lanz, Christa and Bratt, Goran and Neher, Richard A. and Albert, Jan},
 code = {github.com/neherlab/HIVEVO_reservoir},
 date = {2016-11-15},
 doi = {10.7554/eLife.18889},
 issn = {2050-084X 2050-084X},
 journal = {eLife},
 keywords = {cure, evolution, HIV, infectious disease, microbiology, virus},
 language = {eng},
 month = {November},
 pdf = {brodin_establishment_2016.pdf},
 pmcid = {PMC5201419},
 pmid = {27855060},
 projectpage = {hiv.biozentrum.unibas.ch},
 title = {Establishment and stability of the latent {HIV}-1 {DNA} reservoir.},
 volume = {5},
 year = {2016}
}