Richard A. Neher and Erwin Neher
Microscopy research and technique, vol. 64, 185--195, 2004
10.1002/jemt.20078
Abstract
Forster resonance energy transfer (FRET) allows one to study interactions between two fluorescently labeled molecules (donors and acceptors) at distances on the order of 5 nm. Many studies have described methods of how to measure the efficiency of FRET. However, few have addressed the question of how fluorescence from unpaired donors and acceptors can be determined in addition to that from