PoissonNMF is a ImageJ plugin that allows to decompose lambda stacks of fluorescently labeled samples into the contribution of the individual dyes, even if the spectra of the dyes are not or only approximately known.
The method is described in
Neher et al., Biophysical Journal. In addition to blind unmixing, the plugin can also be used for standard or non-negative unmixing.
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Documentation and Examples
The plug-in: PoissonNMF.jar, Version 0.8.7
Links and related pluginsImageJ: rsbweb.nih.gov/ij
Linear unmixing plugin by Joachim Walter: Spectral Unmixing Plugins
2009/02/25 (version 0.8.7)
Implemented the data visualization feature "Simplex projection".
2009/02/18 (version 0.8.6)