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PoissonNTF - fluorescence microscopy data combining multiple excitations

PoissonNTF is a ImageJ plugin, similar to PoissonNMF, that allows to decompose lambda stacks of fluorescently labeled samples into the contribution of the individual dyes. In contrast to PoissonNMF, the NTF version allows to combine spectrally resolved data acquired using different excitation wavelength. The method is described in Neher et al., Biophysical Journal.
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Documentation and Examples

HTML documentation
Tutorial using an example with three dyes imaged on a Leica SP2

Download

The plug-in: PoissonNTF.jar, Version 0.5.0

Authors

Richard Neher
Fabian Theis
Andre Zeug

Please address comments and question to Richard Neher and notify us of any bugs you find.
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Links and related plugins

ImageJ: rsbweb.nih.gov/ij
Linear unmixing plugin by Joachim Walter: Spectral Unmixing Plugins


Published

Oct 19, 2017

Category

software

Tags

  • image analysis 8
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